德州驴NCAPG-DCAF16基因区域多态性与生长性状的关联分析

doi:10.11843/j.issn.0366-6964.2019.02.008

Abstract

Abstract:

The study aimed to investigate the genetic polymorphisms of NCAPG-DCAF16 region in Dezhou donkeys and their associations with growth traits, which can help to mine significant genetic markers for molecular marker assisted selection of Dezhou donkeys. The polymorphisms of NCAPG-DCAF16 region in Dezhou donkeys were detected by DNA pool sequencing technology. Based on the results of DNA pool sequencing, mass-array system was used to genotype the detected SNPs in 227 Dezhou donkeys. Association analysis between the SNPs of NCAPG-DCAF16 region and the growth traits at birth, 3 months old and 6 months old were performed. The results showed that:1) Four SNPs were detected in NCAPG gene, rs1 (T>C) in the 9th intron, rs2 (A>G) in the 11th intron, rs4 (C>G) in the 19th intron, and rs5 (C>G) in the 8th intron. A synonymous mutation was found in the 2nd exon of DCAF16 gene, and the site was rs008 (A>G). 2) The correlation analysis at birth revealed that the average rump length of the individuals with CC genotype at the rs1 was significantly higher than those with CT genotype at birth (P<0.05), the phenotypic values of individuals with different genotypes at 3 months old had no significant difference (P>0.05). At 6 months old, the average chest width of individuals with TT genotype was extremely significantly different from those with CC genotype (P<0.01), and the average shin circumference of individuals with TT and CT genotypes was significantly higher than those with CC genotype (P<0.05). 3) The correlation analysis of rs4 locus indicated that the average chest depth of individuals with GG genotype was significantly higher than those with GC genotype at birth (P<0.05). At 3 months old, the average phenotypic values of individuals with different genotypes was not significantly different (P>0.05). The average chest width and shin circumference of individuals with CC genotype at the age of 6 months were significantly higher than those with GG genotype (P<0.05). 4) The results of rs008 locus correlation analysis showed that there was no significant difference in phenotypic values among individuals with different genotypes at birth (P>0.05). The body weight, body height and rump height of individuals with AA and GA genotypes were significantly higher than those with GG genotypes at the age of 3 months (P<0.05). At 6 months old, the body height and chest circumference of individuals with AA and GA genotypes were extremely significantly higher than those with GG genotype (P<0.01). The difference in chest depth and rump height between individuals with GA and GG genotypes was extremely significantly different (P<0.01). The rump width of individuals with GA genotype was significantly higher than those with GG and AA genotypes (P<0.05). 5) According to the result of linkage disequilibrium analysis, rs1, rs2, rs4 and rs5 had 5 combination genotypes in Dezhou donkeys. The results of association analysis displayed that the average chest width of individuals with TTAACCCC genotype was significantly higher than those with CCGGGGGG genotype at 6 months old (P<0.05). In summary, the 5 SNPs in the NCAPG-DCAF16 region of the Dezhou population have the significant effects on growth traits, especially the rs008 locus has a significant correlation with the important growth traits such as body weight and body height, which can be used as genetic markers to improve the breeding efficiency in Dezhou donkeys.

CLC Number:

S822.2

HOU Haobin, LI Haijing, YANG Li, ZHANG Xinhao, SHI Tianpei, WANG Xinyue, ZHAO Zhida, ZHANG Li. Association between NCAPG-DCAF16 Region Polymorphisms and Growth Traits in Dezhou Donkeys[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 302-313.

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Association between NCAPG-DCAF16 Region Polymorphisms and Growth Traits in Dezhou Donkeys

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